Processes causing a distortion in body fluid mechanics, such as in heart failure or nephrotic. This volume deals with the analyses of body fluids other than blood and urine. The analysis of body fluids, including nucleated blood cell count and differential count, can provide valuable diagnostic information. Access is limited according to attached procedures. One option that has enabled labs to overcome the labor burdens body fluid analysis testing presents has been running body fluid cell counts on hematology analyzers. To protect you from colonization or infection with patients harmful germs and to protect the healthcare environment from germ spread when. Fluid a fluid is a non solid substance such as liquid or gas that tends to flow or conform to the shape of the container. Cell counting body fluids automated body fluid analysis hematology analyzers. Normal cells in all fluids are lymphocytes, macrophages, and lining cells. The evaluation of body fluids typically includes gross examination, microscopic examination, cell and differential counts, chemical analysis. Clean the contaminated equipmentarea immediately with soap and water followed by disinfections with a freshly made solution of 1 part bleach to 10 parts water. Laboratory testing can be performed on many types of body fluids other than blood.
Potentially interfering substances may originate from the following endogenous and exogenous sources. Keep the cup as close to body temperature as possible by wrapping in a clean dry washcloth. The fluid may be sent to a laboratory for testing pleural fluid analysis. For example, the food safety sop cleaning and disinfecting body fluid spills seal the waterproof container with a lid and label with the date.
Therefore, although inulin was the original reference method. Procedures and principles for method verification of standard fluids should be. The evaluation of body fluids typically includes gross examination, microscopic examination, cell and differential counts, chemical analysis and microbiology studies. Cpt coding for microbiology and virology procedures often cannot be determined before the culture is performed. Establishing quality control qc lab limits verification of body fluid analysis if applicable verification of retic results if applicable. New chapters have been added, including a chapter on urine and specialized body fluids. It is also necessary so that a microbiology specimen is not contaminated. Abstract requests for testing various analytes in serous fluids e. Urinalysis and body fluids, 6th edition laboratory. Evaluation of cell count and classification capabilities. Automated body fluids analysis has improved accuracy and precision and is more rapid and less laborintensive than manual cell counts.
Refrigerator 28o c 1 hour for csf, 3 days for body fluids. Body fluid identification is a key component in the forensic scientists tool box and has been carried out both at the crime scene and in the laboratory for many years. To view a list of clsi documents helpful for covid19 testing click here. Blood whole blood, serum, or plasma, body fluids, and bacterial cultures are used in the student laboratories and are considered potentially infectious materials. If your doctor recommends this procedure, heres what to expect. Learn urinalysis and body fluids with free interactive flashcards. Often, these fluids are tested instead of blood because they can give more direct answers to what may be going on in a particular part of the body. From manual microscopy to automated cell counters for.
The xn series automated hematology analyzer has been equipped with a body fluid bf mode to count and differentiate leukocytes in bf samples including cerebrospinal fluid csf. Pleural effusion occurs when fluid collects between the parietal and visceral pleura. Peritoneal fluid present within the 2 layers of peritoneal membrane that cover the abdominal wall and most of the abdominal organs. Laboratory testing can be performed on many types of fluids from the body other than blood. Seminal fluid collection procedure gwinnett medical center. The asssociation of clinical biochemists in ireland the biochemistry of body fluids version 1. Postprocedure dressing gauze and tape or bandaid are sufficient. Csf fluid is obtained to assist in diagnosing central nervous system infection, tumors or vascular accidents. Labcorp test details for body fluid culture, sterile, routine. Post procedure dressing gauze and tape or bandaid are sufficient.
Choose from 500 different sets of urinalysis and body fluids flashcards on quizlet. Pdf in evaluation of ethyl alcohol ea effects, appropriate specimens are required. Cerebrospinal fluid analysis csf is the fluid surrounding the brain and spinal cord. Icsh guidelines for the verification and performance of automated cell counters for body fluids, int. Bloodbody fluid exposure option centers for disease. Body fluids other than blood and urine will be analyzed according to. Fluid accumulation in the pleural, pericardial, and peritoneal cavities is known as a serous effusion. Pdf evaluation of body fluid mode of sysmex xn9000 for.
These exceptions to standard practice must be clearly defined in the sites standard operating procedures. The purpose and practicality of body fluid testing and. Educational commentary differential cell count on cerebrospinal fluid cont. Additionally, crystals in synovial fluids may cause a falsely increased count and may need to be confirmed with the manual count. This guideline provides information for the medical laboratory for evaluating measurement procedures, as well as a strategy to characterize assay performance, when applied to body fluid matrixes. Body fluid collection guidelines, excludes cerebrospinal fluid csf all body fluids must be taken to the laboratory immediately after collection. Synovial fluid analysis is used to help diagnose the cause of joint inflammation.
Jan 01, 2009 the morphology of nucleated cells is the most important diagnostic factor in any body fluid analysis. The remaining chapters cover specific body fluid types and their unique features. List the order of chambers on the air mix temperature control module amtc from left to. Who laboratory manual for the examination of human. Nov 07, 2014 contents introduction amniotic fluid semen analysis synovial fluid gastric fluid other body fluids. Who laboratory manual for the examination and processing. Urinalysis and body fluids, 6th edition laboratory medicine. Includes urine,fluids in the body cavity, joint fluid,sweat, semen etc. Semen is the fluid that comes out of the penis during sexual activity. Npu 28840 pleural fluidnucleated cells body fluid analysis. Gram stain 008540 is recommended with all body fluid cultures additional charge. Key concepts that apply to the entire test cycle, including preexamination, examination, and postexamination phases of body fluid testing, are discussed. For a more detailed explanation of body fluids, consult a hematology or urinalysis textbook that includes a discussion of body fluids, such as hematology.
Background information body fluid analysis is a broad term and refers to tests that may be performed on a sample of body fluid, besides blood. The number is then multiplied by an appropriate factor to obtain the number of erythrocytes and leukocytes per cm3 of body fluid. Validating the performance of body fluid specimens. To overcome this limitation, a new flow cytometrybased technology. Body fluid analysis, including semen analysis automated general chemistry blood gas analysis therapeutic drug monitoring screening for drugs of abuse urinalysis dipstick and microscopy, manual and automated methods microbiology specimen setup, direct specimen examination, stains, and antigen typing for various.
Conclusion the differential cell count is an important component of csf analysis that should always be performed if the total wbc count is elevated. However, its diagnostic accuracy is not reliable for csf samples with low cell concentration at the border between normal and pathologic level. Anyone not involved with administering first aid or cleanup will exit the area to avoid contact with blood and other potentially infectious materials. Ankush explains about synovial fluid, its usage, test procedure and how test results are. Analysis of fluids, such as urine, semen, or sweat.
Body fluid testing for a number of analytes has been reported in the literature. Page 1 of 2 what is semen analysis and why is it tested. The format of each chapter is clear, concise, and easy to follow. Synthesis in the body as a result of oxidation of carbohydrates, adding about 200 mlday daily loss of body water 1. This evalua tion encompasses many disciplines, involves multiple steps, and requires the integration of numerous data elements into the analysis f1. A change in concentration or composition of a particular biochemical constituent in body fluids is used as an indicator of a physiological or pathological condition.
To identify the underlying cause of this accumulation, physicians commonly localize and remove fluid burden, send fluid samples to clinical laboratories for analysis, and offer insight at key points throughout the testing process as part of the diagnostic and prognostic evaluation. We also demonstrate that the automated body fluid analysis on the xe5000 is an acceptable alternative to the microscopic reference method as far as ascitic fluid, peritoneal dialysis fluid, sf or. Who laboratory manual for the examination and processing of human semen 5th ed. American proficiency institute 2018 2nd test event 5 precision, analytic measuring range, analytic specificity, and analyte stability. In addition, any item that comes in contact with these infectious materials blood, body fluids, or. Hand the specimen directly to the laboratory personnel do not leave on counter. This chapter is not intended as a comprehensive treatment of all body fluids, but it covers cell counting and morphologic hematology. Sample should be placed in numbered glass or plastic tubes for csf using order of draw for sequence. Semen is the fluid containing sperm plus other sugar and protein substances thats. I n health care, the analysis of body fluids plays an important role in the diagnosis and management of a wide variety of conditions. Evaluation of body fluids may include total volume, gross appearance, total cell count, differential cell count, identification of crystals, biochemical analysis.
The biochemistry of body fluids association of clinical. See individual test procedures for lists of reagents, supplies, and equipment to be used for testing. Body fluid collection guidelines, excludes cerebrospinal. An automated approach to body fluid analysis medical. Traditionally, clinical laboratorians have provided analysis of body fluid specimens without question while also recognizing the sometimes difficult work. Review of your food service operation to find areas. Includes the anatomy and physiology of the kidney, physical, chemical and microscopic examination of urine, cerebrospinal fluid, and other body fluids as well as quality control, quality assurance and safety. Handle all reagents, controls, and contaminated supplies as potentially biohazardous and use standard precautions in accordance with hospital and laboratory safety and infection control policies. Body fluid cell counts by automated methods clinics in. In this lesson, we explore the information that can be gleaned from synovial and serous fluids, semen and feces, and the means by. Oct, 2016 synovial fluid analysis test checks for presence of bacterial infection in an inflamed joint. Body fluids may be collected in glass or plastic tubes using appropriate anticoagulant if necessary for analysis. Validation of the body fluid module on the new sysmex xn for counting blood cells in cerebrospinal. Semen analysis, also known as a sperm count test, analyzes the health and viability of a mans sperm.
Proper eval uation of csf depends on knowing which tests to order, normal ranges for the patients age. Extent of agreement between the body fluid model of sysmex xn. In some body fluids, the proper order of draw is important the incidence of cellular contamination from tube to tube is reduced. Samples are usually obtained through collection of the fluid in a container e. A semen analysis is a test that looks at the number and motility of sperm. Healthcare providers can benefit from extracting some of the fluid and sending it for analysis. Clinical utility and applicability of published studies to guide interpretation of todays laboratory testing in serous fluids. When performing a shutdown procedure, where is the shutdown button located.
Clinical principles and applications or fundamentals of urine and body fluid analysis. Thoracentesis indications, thoracentesis procedure. This standard operating procedure sop should be implemented to safely and properly respond to all incidents requiring cleaning and disinfecting of body fluid spills. I find the textbook urinalysis and body fluids to be well written and of excellent quality. By jeri walters, shascp a basic understanding of where body fluids come from, why they are there and whats normal is essential for every laboratorian who shoulders the responsibility for testing them. Jul 11, 2017 synovial fluid analysis is used to help diagnose the cause of joint inflammation. There are many types of fluid contained within the human body. Often, these fluids are tested instead of blood because they. Laboratory standard operating procedures, general requirements facilities.
C49ed2 analysis of body fluids in clinical chemistry, 2nd. Cleaning sanitizing after a body fluid blood spill. Extravascular body fluid analysis is compromised by several. Although the correlations concerning cellular component analysis between the sysmex xn. Hematology performance verification manual 18 of 10 pn 4277076ab all procedures will not be completed while your applications specialist is on site. The use of the bloodbody fluid exposure and exposure management options will allow the participating nhsn site to estimate the nature, frequency, circumstances, and sequelae of occupational exposures to bloodborne pathogens i. Wear rubber or latex gloves and splash proof eyewear while cleaning body fluids. Procedures for handling blood and body fluids do not perform first aid or cpr unless you are certified section a. The strengths of each chapter are the understandable learning objectives, key terms, case studies. Blood aspiration sensor not used minimum sample volume required for analysis should be checked by the operator, high risk of wrong count. In addition, hemolyzedand clottedspecimens are not recommended as specimens of choice for analysis. Body fluid analysis for cellular composition bourner g et al. Cerebrospinal fluid analysis csf is the fluid surrounding the brain and.
Dec 01, 2016 a wide variety of diseases and pathological processes cause abnormal accumulation of fluid within the body. Automated cerebrospinal fluid cell counts using the sysmex xe. A chest xray or ultrasound will be done before and after the thoracentesis procedure. Automated body fluid cell counting was initially implemented in 2006 using the sysmex xe2100 sysmex america, mundelein, il. Analysis of body fluids in clinical chemistry clsi. Hemocytometer counts and manual differentials are normally performed on synovial. The strengths of each chapter are the understandable learning objectives, key terms, case studies, clinical situations depicted, and technical tips. Additionally, saliva testing is becoming more common for some laboratory testing, such as hiv testing, drugs abuse testing and genetic tests. Educational commentary is provided through our affiliation with the american society for clinical.
A table of factors affecting analysis is included for easy reference. Body fluids in the hematology laboratory oncohema key. Traditionally, clinical laboratorians have provided analysis of body fluid specimens without question while also recognizing the sometimes difficult work that goes into their collection. Appropriate laboratory testing and specimen utilization is therefore. This atlas addresses primarily the elements of fluids that are observable through a microscope. The hematology laboratory of university hospitals case medical center, cleveland, oh, performs approximately 325 csf and 150 noncsf body fluid analyses monthly. Food safety sample sop cleaning and disinfecting body fluid spills purpose.
While some cells may be found in all fluids, their significance may be different depending on the fluid being analyzed. Thoracentesis indications, thoracentesis procedure, position. Body fluids including vomit, diarrhea, and blood are considered potentially infectious. Comparative analysis was performed using controls having an equal volume of deionized water and body fluid specimen. With the release of the second edition of clsis guideline, analysis of body fluids in clinical chemistry, laboratories will have guidance to embark on a body fluid validation project to verify an assays performance in body fluid matrices and clinical utility of the testing menu 1, 2, 6. The analysis of body fluids plays a critical role in the diagnosis and prognosis of a disease. Body fluid specimens were spiked with known varying concentrations of the analyte from either a standard solution, calibration verification material, or serum with a known concentration.
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